Jovialis
Advisor
- Messages
- 9,890
- Reaction score
- 6,794
- Points
- 113
- Ethnic group
- Italian
- Y-DNA haplogroup
- R1b-PF7566>Y227216
- mtDNA haplogroup
- H6a1b7
Genome-scale sequencing and analysis of human, wolf, and bison DNA from 25,000-year-old sediment
Highlights
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A single shotgun-sequenced Pleistocene sediment yielded genomic data of three mammals
•
Sediment genome sequencing can produce data comparable to that from skeletal remains
•
A pre-LGM human lineage from the Caucasus was an ancestral component of West Eurasia
•
∼0.01X wolf and bison environmental genomes, suggesting reshaping of populations
Cave sediments have been shown to preserve ancient DNA but so far have not yielded the genome-scale information of skeletal remains. We retrieved and analyzed human and mammalian nuclear and mitochondrial environmental “shotgun” genomes from a single 25,000-year-old Upper Paleolithic sediment sample from Satsurblia cave, western Georgia:first, a human environmental genome with substantial basal Eurasian ancestry, which was an ancestral component of the majority of post-Ice Age people in the Near East, North Africa, and parts of Europe; second, a wolf environmental genome that is basal to extant Eurasian wolves and dogs and represents a previously unknown, likely extinct, Caucasian lineage; and third, a European bison environmental genome that is basal to present-day populations, suggesting that population structure has been substantially reshaped since the Last Glacial Maximum. Our results provide new insights into the Late Pleistocene genetic histories of these three species and demonstrate that direct shotgun sequencing of sediment DNA, without target enrichment methods, can yield genome-wide data informative of ancestry and phylogenetic relationships.
https://www.sciencedirect.com/science/article/abs/pii/S0960982221008186
Highlights
•
A single shotgun-sequenced Pleistocene sediment yielded genomic data of three mammals
•
Sediment genome sequencing can produce data comparable to that from skeletal remains
•
A pre-LGM human lineage from the Caucasus was an ancestral component of West Eurasia
•
∼0.01X wolf and bison environmental genomes, suggesting reshaping of populations
Cave sediments have been shown to preserve ancient DNA but so far have not yielded the genome-scale information of skeletal remains. We retrieved and analyzed human and mammalian nuclear and mitochondrial environmental “shotgun” genomes from a single 25,000-year-old Upper Paleolithic sediment sample from Satsurblia cave, western Georgia:first, a human environmental genome with substantial basal Eurasian ancestry, which was an ancestral component of the majority of post-Ice Age people in the Near East, North Africa, and parts of Europe; second, a wolf environmental genome that is basal to extant Eurasian wolves and dogs and represents a previously unknown, likely extinct, Caucasian lineage; and third, a European bison environmental genome that is basal to present-day populations, suggesting that population structure has been substantially reshaped since the Last Glacial Maximum. Our results provide new insights into the Late Pleistocene genetic histories of these three species and demonstrate that direct shotgun sequencing of sediment DNA, without target enrichment methods, can yield genome-wide data informative of ancestry and phylogenetic relationships.
https://www.sciencedirect.com/science/article/abs/pii/S0960982221008186
